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Something is fishy about Subway’s “tuna” sandwich. Commercial lab tests again found no identifiable tuna DNA in the sandwich that purports to contain the fish. The New York Times bought Subway tuna sandwiches from three different locations in Los Angeles, and then sent frozen samples to an unidentified commercial food testing lab after two California women filed a class-action lawsuit against the company in January alleging that Subway’s tuna sandwiches aren’t actually made of the fish. The women ordered tuna from the sandwich giant at locations near their home, but “independent testing has repeatedly affirmed, the products are made from anything but tuna,” the suit said. The lab the Times used to test the tuna most recently declined to be identified for fear of losing any opportunities to work directly with Subway, the country’s largest sandwich chain, the outlet reported. The newspaper said it paid about $500 for the lab, which specializes in fish testing, to conduct a polymerase chain reaction, or PCR, test to see if the substance had any of five different tuna species. PCR tests rapidly replicate huge amounts of a specific DNA sample.

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